Simplified apparatus for ultrafiltration by centrifugation.

Simple ultrafiltration apparatus.

All ultrafiltration devices have in common a semipermeable membrane and a means for creating a pressure difference across it. They differ only in the configuration of the membrane, the nature of the force used to create a pressure difference, and the ease with which the environment of the plasma can be controlled so as to simulate physiological conditions. Speed of producing ultrafiltrate provi...

Concentration and purification of poliovirus by ultrafiltration and isopycnic centrifugation.

A method is described by which poliovirus can be rapidly and simply concentrated by the use of a Diaflo XM-50 ultrafilter membrane. Freon-extracted ultrafilter concentrates banded in CsCl provided a 1,724-fold volumetric concentration of poliovirus. During concentration, trypsin-digested cellular material can pass through the ultrafilter membrane, thus providing a versatile means of degrading a...

A Simplified Apparatus for Photometric Analysis and Photomicrography

A significant advance in the technique of histology and cytology is the current trend toward the use of quantitative methods of studying microscopical preparations; in other words, of substituting an objective measure of absorption 'for expressions like "more basophilic," less Feulgeu-positive," etc. Among such methods are those which Caspersson has developed for determining the absorption spec...

Ultrasound-Assisted Extraction, Centrifugation and Ultrafiltration: Multistage Process for Polyphenol Recovery from Purple Sweet Potatoes.

This work provides an evaluation of an ultrasound-assisted, combined extraction, centrifugation and ultrafiltration process for the optimal recovery of polyphenols. A purple sweet potato (PSP) extract has been obtained using ultrasonic circulating extraction equipment at a power of 840 W, a frequency of 59 kHz and using water as solvent. Extract ultrafiltration, using polyethersulfone (PES), wa...

Sucrose gradient centrifugation sedimentation standards: a simplified technique.